1/27/2024 0 Comments Imagej auto threshold![]() ![]() If you aren’t happy with the results obtained from you images, you need to change sample preparation, image acquisition and perhaps pre-processing. You should not change the size limits after you have decided for using certain limits. The picture I shared is just a cropped image from from original picture) Note: We capture images with Moticam DS Scanner (with built in Moticam Pro camera), the original images are in MDS File formate (can only be open with Motic DSAssistant Software) with original size that may range from 10MB to 40MB based on size of the sample on slide. I would therefore be eager to listen from you in a step wise manner exactly what you did in last image. Now in afterwards steps I am completely blind, I tried different steps but can’t repeat what you did for me in last attached pictures,ĥ) Image>threshold/Auto threshold(tried both individually)>ApplyĦ) Image>threshold>Apply (I always do this step because I learn by reading to tell image J to measure the threshold area)ħ)Ěnalyze>Set Measurement> (Area, Area Fraction, limit to threshold, Display label and Redirect to None were selected)>OkĪnd then… every thing goes wrong at the end Multipoint tool> (all points were labeled/selected)>Ok.Though I am a beginner of Fiji but I tried myself to repeat what you most probably have done. This is exactly what I am in need and I would very much like to know the steps involved to make this measurement. Yes! you are right I am interested in obtaining the percentage of dark brown to black structure in the red region.
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